DNase I (RNase-free) from New England Biolabs

Product Specs
ItemDNase I (RNase-free)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0303L
Concentration1,000 units/ml
Quantity5000 units
ApplicationsFunctional Genomics
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer.

Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A robust CRISPR interference gene repression system in Vibrio parahaemolyticusArch MicrobiolDecember 26, 2023Taoyuan Jiang, Yuhuan Li, Wencong Hong, Mingyu Linwas extracted using hot phenol–chloroform. Total RNA samples were then treated with DNase I (NEB, Cat#M0303S), and equal amounts of RNA were reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit

(2) Cell-Free RNA Signatures Predict Alzheimer’s DiseaseiScienceDecember 15, 2023Alejandro Cisterna-García, Aleksandra Beric, Muhammad Ali, Jose Adrian Pardo, Hsiang-Han Chen, Maria Victoria Fernandez, Joanne Norton, Jen Gentsch, Kristy Bergmann, John Budde, et al.peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, DNAseI,NEB,Cat# M0303 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Maxwell®

(3) Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus ErythematosusCurr Issues Mol BiolDecember 8, 2023Tatiana S. Novikova, Evgeny A. Ermakov, Elena V. Kostina, Alexander N. Sinyakov, Alexey E. Sizikov, Georgy A. Nevinsky, Valentina N. Bunevaanalysis of ODN hydrolysis by IgG antibodies or the control enzyme deoxyribonuclease I (DNase I) (Cat #: M0303S, New England Biolabs, Ipswich, MA, USA) was carried out in a reaction mixture (70 μL) containing 50

(4) Teneurin-3 regulates the generation of non-image-forming visual circuitry and responsiveness to light in the suprachiasmatic nucleusPLoS BiolDecember 4, 2023John L. Hunyara, K. M. Daly, Katherine Torres, Maria E. Yurgel, Ruchi Komal, Samer Hattar, Alex L. Kolodkinphenol red). Cells were again centrifuged and resuspended in 2,000 U/ml DNase I (New England Biolabs, M0303S) in Hibernate A medium (lacking Ca2+, Mg2+, and phenol red) prior to cell sorting. Individual GFP+,

(5) Mapping and modeling human colorectal carcinoma interactions with the tumor microenvironmentNature CommunicationsNovember 30, 2023Ning Li, Qin Zhu, Yuhua Tian, Kyung Jin Ahn, Xin Wang, Zvi Cramer, Justine Jou, Ian W. Folkert, Pengfei Yu, Stephanie Adams-Tzivelekidis, et al.fragments settling down at the bottom of the tube are collected and minced again. DNase I (NEB Order no. M0303L) is then added to the dissociation solution to the final concentration of 10 U/ml (200X) and incubated

(6) Biomolecular condensates in fungi are tuned to function at specific temperaturesbioRxivNovember 30, 2023Benjamin M. Stormo, Grace A. McLaughlin, Logan K. Frederick, Ameya P. Jalihal, Sierra J Cole, Ian Seim, Fred S. Dietrich, Amy S. Gladfelterfor 1hr. The solution was diluted to 50ul and then the DNA was digested by addition of 2ul of DNAse (M0303, New England BioLabs, Waltham, MA). The RNA was precipitated by addition of 25µl of 2.5M LiCl and then

(7) Uncoupling the distinct functions of HP1 proteins during heterochromatin establishment and maintenanceCell ReportsNovember 28, 2023Melissa Seman, Alexander Levashkevich, Ajay Larkin, Fengting Huang, Kaushik RagunathanMembrane,GE Healthcare,10600022 EMM powder,Formedium,PMD0402 Yeast extract,Gibco,2911929 DNase I,NEB,M0303 "Phenol chloroform, acidic",VWR,E277 Adenine hemisulfate salt,Chem-Impex Int’l,00006 Streptavidin magnetic beads

(8) Sis2 regulates yeast replicative lifespan in a dose-dependent mannerNature CommunicationsNovember 27, 2023Tolga T. Ölmez, David F. Moreno, Ping Liu, Zane M. Johnson, Madeline M. McGinnis, Benjamin P. Tu, Mark Hochstrasser, Murat AcarrYeast Total RNA kit (IBI #IB47410), following the manufacturer’s instructions. DNAse treatment (NEB, #M0303S) was performed in column. RNA concentration and purity were evaluated in a NanoDrop 2000 (Thermo Scientific

(9) Detection of isoforms and genomic alterations by high-throughput full-length single-cell RNA sequencing in ovarian cancerNature CommunicationsNovember 27, 2023Arthur Dondi, Ulrike Lischetti, Francis Jacob, Franziska Singer, Nico Borgsmüller, Ricardo Coelho, Rudolf Aebersold, Melike Ak, Faisal S. Al-Quaddoomi, Silvana I. Albert, et al.solution (1 mg/mL collagenase/Dispase [Sigma cat. no. 10269638001], 1 unit/mL DNase I [NEB, cat. no. M0303] and 10% fetal bovine serum (FBS) in DMEM [Sigma, cat. no. D8437-500mL]) for 30 min at 37 °C. To focus

(10) Single-cell epigenomics and spatiotemporal transcriptomics reveal human cerebellar developmentNature CommunicationsNovember 22, 2023Suijuan Zhong, Mengdi Wang, Luwei Huang, Youqiao Chen, Yuxin Ge, Jiyao Zhang, Yingchao Shi, Hao Dong, Xin Zhou, Bosong Wang, et al.tissue was first digested in 2 mg/ml collagenase IV (Gibco, Cat.17104-019) and 10 U/μl DNase I (NEB, Cat.M0303L) in hibernate E medium and then 1 mg/ml papain (Sigma, Cat.P4762) and 10 U/μl DNase I in hibernate E

(11) Profiling stress-triggered RNA condensation with photocatalytic proximity labelingNature CommunicationsNovember 15, 2023Ziqi Ren, Wei Tang, Luxin Peng, Peng Zoumanufacturer’s instructions (~700–1000 μg from HEK293T cells in a 15-cm dish), digested with 1-2 μL DNaseI (NEB, M0303) at 37 °C for 30 min, and then incubated with click reagents consisting of 0.1 mM biotin-azide, 2 mM

(12) Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probesNature CommunicationsNovember 14, 2023Ren Ren, Shenglin Cai, Xiaona Fang, Xiaoyi Wang, Zheng Zhang, Micol Damiani, Charlotte Hudlerova, Annachiara Rosa, Joshua Hope, Nicola J. Cook, et al.µl, followed by incubating at 37 °C for 2 h. The product was further treated with 1 µl of DNase I (M0303S, New England Biolabs), 2 µl of DNase I reaction buffer, and 2 µl of Nanopure water to remove any remaining

(13) Unistrand piRNA clusters are an evolutionarily conserved mechanism to suppress endogenous retroviruses across the Drosophila genusNature CommunicationsNovember 13, 2023Jasper van Lopik, Azad Alizada, Maria-Anna Trapotsi, Gregory J. Hannon, Susanne Bornelöv, Benjamin Czech NicholsonScientific; catalogue nr. 15596026). RNA was treated with DNase (New England BioLabs; catalogue nr. M0303) followed by ribosomal RNA depletion using RiboPOOL (siTOOLs Biotech; catalogue nr. dp-K024-000007)

(14) PRMT2 promotes HIV-1 latency by preventing nucleolar exit and phase separation of Tat into the Super Elongation ComplexNature CommunicationsNovember 10, 2023Jiaxing Jin, Hui Bai, Han Yan, Ting Deng, Tianyu Li, Ruijing Xiao, Lina Fan, Xue Bai, Hanhan Ning, Zhe Liu, et al.polymerase chain reaction Total RNA was isolated using TRIzol, treated with deoxyribonuclease I (New England Biolabs, M0303S), and reverse transcribed using SuperScript III and random primers (Invitrogen). The resulting cDNA

(15) Spatial and single-cell profiling of the metabolome, transcriptome and epigenome of the aging mouse liverNature AgingNovember 9, 2023Chrysa Nikopoulou, Niklas Kleinenkuhnen, Swati Parekh, Tonantzi Sandoval, Christoph Ziegenhain, Farina Schneider, Patrick Giavalisco, Kat-Folz Donahue, Anna Juliane Vesting, Marcel Kirchner, et al.min at 300 g. The pellet was incubated on ice for 20 min in EZ-buffer supplemented with DNAseI NEB M0303S (4 U ml−1) and 1X DNAseI buffer. Equal volume of EZ-buffer was added and samples were spun 5 min at

(16) Specific heterozygous variants in MGP lead to endoplasmic reticulum stress and cause spondyloepiphyseal dysplasiaNature CommunicationsNovember 3, 2023Ophélie Gourgas, Gabrielle Lemire, Alison L. Eaton, Sultanah Alshahrani, Angela L. Duker, Jingjing Li, Ricki S. Carroll, Stuart Mackenzie, Sarah M. Nikkel, Michael B. Care4Rare Canada Consortium, et al.HEK-293 cells with TRIZOL reagent (Invitrogen #15596026) and subjected to DNase I (New England Biolabs #M0303S) treatment. The first- strand cDNA synthesis and qRT-PCR were performed using a High-Capacity cDNA Reverse-

(17) Defining early steps in Bacillus subtilis biofilm biosynthesismBioOctober 31, 2023Christine A. Arbour, Rupa Nagar, Hannah M. Bernstein, Soumi Ghosh, Yusra Al-Sammarraie, Helge C. Dorfmueller, Michael A. J. Ferguson, Nicola R. Stanley-Wall, Barbara Imperialimg lysozyme (Research Products International, cat #L38100); 25 µL DNAse I (New England BioLabs cat #M0303S); and 50 µL protease inhibitor cocktail (Roche, cat #11836170001). Cells were sonicated twice for 1.5

(18) The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epitheliumNature CommunicationsOctober 28, 2023Zhaoqi Shu, Minghe Fan, Bo Tu, Zhiheng Tang, Haojie Wang, Haimeng Li, Hengchao Li, Meng Yuan, Jingru Bai, Sihan Huo, et al.Dispase II (Sigma, #4942078001), 1 mg/ml Trypsin Inhibitor (Sigma, #T6522) and 1 unit/ml DNase I (NEB, # M0303S) for 45 min at 37 °C with agitation. Cell digestion of both tumor and adjacent normal samples were strained

(19) Genome-wide analysis of the interplay between chromatin-associated RNA and 3D genome organization in human cellsNature CommunicationsOctober 16, 2023Riccardo Calandrelli, Xingzhao Wen, John Lalith Charles Richard, Zhifei Luo, Tri C. Nguyen, Chien-Ju Chen, Zhijie Qi, Shuanghong Xue, Weizhong Chen, Zhangming Yan, et al.manufacturer’s instructions. Resulting transcription reactions were treated with DNAse I (New England Biolabs-M0303S), and RNA was purified by Qiagen RNeasy mini spin column (Qiagen-74104) and eluted in RNAse-free water. Phosphatase

(20) Carcinogen exposure enhances cancer immunogenicity by blocking the development of an immunosuppressive tumor microenvironmentJournal of Clinical InvestigationOctober 16, 2023Mei Huang, Yun Xia, Kaiwen Li, Feng Shao, Zhaoyi Feng, Tiancheng Li, Marjan Azin, Shadmehr Demehriprepared after Collagenase IV (catalog no. LS004189, Worthington Biochemical) and DNase I (catalog no. M0303S, New England Biolabs) digestion and tumor infiltrating immune cells were enriched with CD45 MicroBeads

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